2-day The HPLC & LC-MS Clinic

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Course summary

  • Number of days: 2
  • Experience level: Intermediate
  • Type: Generic, applicable to all instrumentation
  • Teaching method: Face-to-face classroom
  • Course venue: The Open University, Milton Keynes, UK | See individual dates for other venues
  • Onsite/offsite availability: Arrange this course just for you
  • Scheduled course price: £530 + VAT per delegate

The HPLC & LC-MS Clinic is often taken as a follow-on course from the Practical Essentials of HPLC & LC-MS, either during the same week or at a later stage, and together they form the Complete HPLC & LC-MS course.

What will you learn on this course?

First, the course examines the method development process in detail; covering the objectives of analytical method, how many components to be separated and the required sensitivity, selectivity, specificity and the speed of analysis. This is followed by system optimisation and configuration for LC and LC-MS. All of this knowledge is then put to the test in case studies.

The course then looks at method validation protocols, system suitability and outlines the steps required for analytical method accreditation.

The last part of the day covers a brief introduction to some advanced techniques that can be used for difficult or complex samples including detector selectivity and specificity, UHPLC vs. HPLC (brief introduction to method transfer), chromatographic peak deconvolution using tandem mass spectrometry, multi-dimensional LC (LCxLC), on-line SPE and chemometrics.

On the second day the course looks at clean-up procedures for difficult samples. The parts of the LC & MS system requiring maintenance, how often and how to identify when maintenance is required are then covered in detail.

Following on from this, we look at preparing for problems, how to identify problems and the troubleshooting process.  This knowledge, along with the basic knowledge of LC & LC-MS acquired in days 1-3 of the Practical Essentials of LC & LC-MS course are then put to the test in a practical exercise on troubleshooting chromatograms through the final part of the day. 

Learning Outcomes

  • You will learn strategies for LC & LC-MS method development.
  • You will learn about system optimisation and configuration for LC and LC-MS.
  • You will get an introduction to LC & LC-MS advanced techniques.
  • You will learn to recognise areas requiring maintenance on the instrument.
  • You will learn the logical steps of instrument troubleshooting.
  • You will troubleshoot problematic chromatograms.

Who is this course for?

If you have reasonable liquid chromatography experience, or have attended the Practical Essentials of LC & LC-MS course, then please join us for this advanced training.

If you would like to improve your knowledge in a particular area and don't want to attend a 2-day course you can choose to attend individual days from The LC & LC-MS Clinic course.

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