Applied LC and LC-MS method development and advanced techniques
- Duration: 1 day for live courses
- Experience level: Intermediate
- Delivery methods: Face-to-face | Offsite training & consultancy | On-Demand | Online | Onsite training & consultancy | Tailored training |
- Type of course: Manufacturer specific
- Applicable to manufacturer(s): JASCO |
- CPD Approved: Not yet approved
- Scheduled course price: £425.00 + TAX per delegate
- Course discounts: RSC CAMS HEaTED Cambridge Network members receive a discount on this course
Your applied guide to method development and advanced analytical techniques for liquid chromatography (LC) and liquid chromatography-mass spectrometry (LC-MS)
Day 4 and modules 9 and 10 of the Complete LC & LC-MS and The LC and LC-MS Clinic course.
Applied LC and LC-MS method development and advanced techniques is approved by the Royal Society of Chemistry (RSC) for the purposes of Continuing Professional Development (CPD), and provides an in-depth exploration of LC and LC-MS method development alongside advanced chromatographic and mass spectrometric techniques used in modern analytical laboratories. Designed for professionals, students, and analytical scientists wishing to expand their practical and theoretical understanding of LC and LC-MS, this training provides both the foundations of effective method development and an introduction to advanced analytical approaches used when conventional methods are no longer sufficient.
This day and modules can be combined with any of the other twelve modules and days in the Complete LC and LC-MS course to tailor your training needs.
Why should I join?
On completion of Day 4 training, you will gain an understanding of LC and LC-MS method development and advanced analytical techniques, giving you:
- An overview of the complete LC and LC-MS method development process from concept through to method validation
- Understanding of how analytical objectives influence method design, detector selection, instrumentation choice, and workflow decisions
- Insight into optimisation strategies for sampling, sample preparation, injection, separation, detection, and data acquisition
- Practical guidance on troubleshooting chromatographic and mass spectrometric challenges
- Insight into mass spectral interpretation and structural elucidation principles
- An introduction to multidimensional chromatography, alternative separation modes, and advanced LC-MS acquisition strategies
- An excellent addition to other modules and days within the Complete LC and LC-MS training programme
What would I learn?
This day focuses on method development and advanced analytical techniques for LC and LC-MS, you will learn:
- How to define analytical objectives and develop a structured workflow for LC and LC-MS method development
- The principles of column selection, including stationary phase chemistry, particle size, pore size, column dimensions, and chiral separations
- How to develop and optimise isocratic and gradient separations for complex sample matrices
- How to optimise detector performance for UV-Vis, fluorescence, and mass spectrometric detection systems
- The principles of mass spectral interpretation and structural identification using LC-MS data
- How isotopic patterns, fragmentation pathways, and accurate mass measurements assist compound identification
- How multidimensional chromatography techniques including heart-cutting LC-LC and comprehensive LC×LC improve separation performance
- The theory and practical applications of HILIC, ion-pair chromatography, ion-exchange chromatography, and size exclusion chromatography
- How advanced detector technologies and acquisition strategies can improve analytical performance and information content
What knowledge will I gain?
By the end of the day, you will:
- Understand the complete process of developing an LC or LC-MS method from initial concept through to routine application
- Be able to identify and optimise the critical parameters affecting chromatographic resolution, sensitivity, selectivity, and robustness
- Understand how to systematically optimise sample preparation, injection, separation, and detection conditions
- Know how to select appropriate columns, mobile phases, detectors, ionisation techniques, and mass analysers for specific analytical applications
- Understand how to maximise sensitivity while minimising matrix effects, contamination, carryover, and analyte loss
- Know how to evaluate and improve analytical method performance through optimisation and validation
- Gain insight into quality assurance procedures including blanks, spikes, replicates, internal standards, and surrogate standards
- Be able to interpret mass spectral information to support compound identification and structural elucidation
- Understand the strengths, limitations, and applications of advanced chromatographic techniques and specialist detector technologies
Is this course for me?
This day is designed for anyone who would like to learn more about LC and LC-MS method development and advanced analytical techniques.
- Laboratory analysts and technicians using LC, HPLC, UHPLC, and LC-MS systems
- Laboratory or line managers responsible for LC and LC-MS instrumentation, analytical strategy, and data quality
- Data analysts and report writers working with LC and LC-MS data
- Service engineers who would benefit from a chemistry-focused introduction to advanced LC and LC-MS applications
- Sales, commercial, or marketing teams working with LC, LC-MS, consumables, instrumentation, analytical services, or laboratory technologies who would like more knowledge
If LC or LC-MS touches your work in any way, this well-planned training day, delivered by experts in the field, equips you with the knowledge required to understand these techniques.
