Absolute Basics of HPLC and LC-MS
- Duration: 1 day for live courses 6.50 hours for on-demand
- Experience level: Beginners
- Delivery methods: Face-to-face | Offsite training & consultancy | On-Demand | Online | Onsite training & consultancy | Tailored training |
- Type of course: Universal
- Applicable to manufacturer(s): Agilent | Bruker | PerkinElmer | Sciex | Scion | Shimadzu | SRI | Thermo | Varian | Waters |
- CPD Approved: Approved
- Scheduled course price: £385.00 + TAX per delegate
- Course discounts: RSC CAMS HEaTED members receive a discount on this course
What will you learn on this course?
Your beginner’s guide to High Performance Liquid Chromatography & Mass Spectrometry
Learn the fundamentals of High Performance Liquid Chromatography (HPLC) and Liquid Chromatography ‑ Mass Spectrometry (LC-MS) with this beginner‑friendly 1-day theory course. Build confidence in chromatography, mass spectrometry and applying your newly learned knowledge to real‑world applications. This HPLC and LC-MS course is Royal Society of Chemistry approved training for purposes of Continuing Professional Development (CPD).
Whether you’re stepping into analytical chemistry for the first time or looking to strengthen your technical expertise, this 1-day essentials training gives you the confidence and clarity you need to understand, and start using, HPLC and LC-MS. These foremost analytical chemistry techniques are used to separate, identify and quantify components in complex mixtures and therefore contribute in most labs. This course breaks complex instrumentation and techniques into easy-to-understand concepts, helping you build skills that you can apply in the lab.
Why Join the Absolute Basics of HPLC and LC-MS?
On completion, you will be able to understand what HPLC and LC-MS instruments do, how they work, and why they are one of the most used analytical chemistry tools today. This course gives you:
- Strong foundational knowledge with no assumed experience
- Clear explanations and examples of chromatography and mass spectrometry principles
- Confidence to start to interpret data and understand what it means
- Insights into real applications across multiple industries using animated applications
- Better understanding of the instrumentation with instrument parts and consumables to see.
- A meaningful certificate for your CPD training, whether you’re in a technical role, a manager or an adjacent industry
This is your opportunity to understand two of the most widely used analytical techniques in modern science.
What You Will Learn on this HPLC and LC-MS
A complete beginner‑friendly introduction focusing on the what, how, why, where and when of HPLC and LC‑MS, including:
What HPLC and LC‑MS actually are
- What is liquid chromatography?
- What is mass spectrometry?
- What is specific to HPLC and its hyphenated technique LC‑MS
How HPLC and LC-MS work
- The core principles behind chromatographic separation
- How mass spectrometers detect and measure analytes
- Instrument components, functions and workflows
Why we use HPLC & LC‑MS
- The problems they solve
- The answers and insights they provide
- Basic qualitative and quantitative data analysis
Where HPLC and LC-MS are applied
- Suitability of types of samples and analytes
When HPLC or LC‑MS is the right choice
- Real examples across pharmaceuticals, environmental science, food testing, forensics, clinical labs, petrochemicals and more
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Learning outcomes
By the end of the course, you will:
- Understand the different types of chromatography
- Clearly define LC, HPLC, MS and LC‑MS, and appreciate the differences
- Know the key components of the liquid chromatograph and mass spectrometer
- Comprehend the principles behind data generation and data analysis
- Be familiar with common sampling and injection techniques
- Recognise how HPLC and LC‑MS are used across diverse industries
- Feel confident discussing instrumentation, workflows and results
- Understand what’s next in the your path of progression for HPLC and LC-MS
- Receive a acronym glossary on HPLC and LC-MS
This course gives you the language and understanding needed to work effectively with chromatographic systems, even if you’re not the person pressing the buttons.
Who is this course for?
This training is designed for anyone who needs a solid grounding in HPLC or LC‑MS, including:
- Complete beginners to chromatography or mass spectrometry
- Analysts & technicians wanting a clear, structured refresher
- Laboratory or line managers overseeing instruments
- Data analysts and report writers who interpret results but don’t use the instruments
- Service and consultant engineers who need a chemistry-focused viewpoint
- Sales, marketing or commercial teams working with HPLC and LC‑MS systems, consumables or laboratory services
If HPLC or LC‑MS touches your work in any way, this well-planned course delivered by experts in the field equips you to understand the science behind the technique with confidence.
Course progression
Feedback from previous attendees
“A really detailed basics course that covered a lot more of the theoretical side of HPLC & LC-MS. I found it very easy to follow along & it was a perfect refresher course for me. It helped me bridge some of the knowledge gap I had, especially about MS.” Delegate, 2026
“Other courses provide a very brief knowledge around different industries, but Anthias has covered almost all examples & the instructor gave an understanding of the variety of samples used in the industry”’ Laboratory Technician, 2026
“Material well structured, presenter went into detailed answers on all the questions & gave loads & loads of examples. We got to see parts of the instruments which was very useful.” Product Development Intern, 2026
“Diane was extremely knowledgeable and shared her knowledge in an easy to understand way.” Delegate, April 2023.
“Although I did not watch the video live, I enjoyed the last part of the course where we should interact in the classroom and suggested answers for the analysis of some analytes.” On-Demand Virtual Classroom: Absolute Basics of HPLC & LC-MS, 2021.
“I’ve enjoyed that the machinery and some parts of the instruments were shown and described (photos and real-life on the tutor desk). I’ve liked taking part in solving the problems with the instructor at the end of the course and talking about real-life examples, where HPLC can be used and what can be tested with it. The slides were very neat and clearly presented with a good amount of images and graphs added.” Live Virtual Classroom: Absolute Basics of HPLC & LC-MS, May 2020.
“I really enjoyed the interactive questions at the end to test our knowledge. I enjoyed learning about quadrupoles the most.” Delegate, April 2023.
“(I enjoyed) the interactiveness of the course, all of the instrument parts that got passed around.” Onsite training, June 2022.
“Engaging course instructor, being able to see and pass around the parts, interesting and good pace.” Onsite training, June 2022.
“Getting hands on with instrument parts – really gives context and further info.” Onsite training, June 2022.
“The breakdown of different sections of the LC and MS rather than viewing them as a whole.” Onsite training, June 2022.
“It was a very good refresher for me personally.” John Adey, Scientist, Environment Agency (February 2018).
