Hands-on UV-Vis
- Duration: 1 day for live courses 6.50 hours for on-demand
- Experience level: Intermediate
- Delivery methods: Face-to-face | Offsite training & consultancy | Onsite training & consultancy | Tailored training |
- Type of course: Universal
- Applicable to manufacturer(s): JASCO | Thermo |
- CPD Approved: Not yet approved
- Scheduled course price: £650.00 + TAX per delegate
- Course discounts: RSC CAMS HEaTED Cambridge Network members receive a discount on this course
Your Hands-on approach to learning UV-Vis Spectroscopy
Combine the practical theory of UV-Visible (UV-Vis) spectroscopy with extensive laboratory-based exercises in this 1-day Hands-on training. Designed to bridge the gap between theory and practice, the course provides a thorough understanding of UV-Vis instrumentation, spectral interpretation, quantitative analysis, method development and routine operation.
Gain practical experience using UV-Vis spectrophotometers while developing an understanding of the fundamental principles that underpin absorption spectroscopy. From electronic transitions and chromophores through to quantitative analysis, calibration development and method validation, the course combines theory with laboratory exercises to build confidence in using UV-Vis as an analytical technique.
This Hands-on UV-Vis course is ideal for analysts, scientists and laboratory professionals who want to understand how UV-Vis works, improve the quality of their results and develop robust analytical methods for routine laboratory applications.
Why should I join?
On completion, you will have both the theoretical knowledge and practical experience required to confidently use UV-Vis instrumentation for qualitative and quantitative analysis.
This Hands-on UV-Vis course gives you:
- Strong theoretical and practical skills for using UV-Vis spectrophotometers
- A clear explanation of light absorption and electronic spectroscopy
- Practical guidance on collecting high-quality UV-Vis data
- An understanding of how molecular structure influences UV-Vis spectra
- Insight into calibration curve development and quantitative analysis
- Guidance on method development and validation principles
- An understanding of instrument performance and routine operation
- A valuable refresher for existing UV-Vis users
What would I learn?
A user-friendly UV-Vis course combining theory with practical laboratory exercises, you will learn:
- The principles of electromagnetic radiation and UV-Visible spectroscopy
- The role of molecular orbitals, structure, chromophores and conjugated systems in UV-Vis absorption
- The strengths and limitations of UV-Vis for qualitative analysis
- The relationship between transmittance, absorbance and the Beer-Lambert Law
- How calibration curves are developed and applied
- The functions of sources, monochromators, gratings, detectors and optical systems
- Practical considerations for sample handling, cell selection and solvent choice
- Techniques for dealing with weak absorbance, strong absorbance and spectral interferences
What knowledge will I gain?
- Understand how UV and visible light interact with molecules
- Be confident discussing the theory and operation of modern UV-Vis spectrophotometers
- Understand electronic transitions, chromophores and conjugation effects
- Explain the relationship between absorbance, concentration and path length
- How to Select suitable optical cells, path lengths and solvents for different applications
- Develop reliable calibration curves and quantitative methods
- Recognise the causes of instrumental and sample-related errors
- Understand the importance of wavelength accuracy, photometric accuracy and instrument stability
- Optimise spectral resolution, signal-to-noise ratio and sensitivity
- Understand correction techniques used to compensate for scattering, baseline drift and interferences
Is this course for me?
This training is designed for anyone who needs a solid understanding of UV-Vis, including:
- Analysts & technicians requiring training or a clear structured refresher of UV-Vis
- Laboratory or line managers overseeing UV-Vis instruments
- Data analysts and report writers who interpret results but don’t use the instruments
- Service and consultant engineers who need both a theoretical and a hands-on reminder
- Sales, marketing or commercial teams working with UV-Vis systems, consumables, data or laboratory services
If UV-VIs touches your work in any way, this well-planned course delivered by experts in the field equips you to understand the science behind these techniques.
