Introduction to advanced LC and LC-MS techniques
- Duration: 3 hours for on-demand
- Experience level: Intermediate
- Delivery methods: Offsite training & consultancy | On-Demand | Online | Onsite training & consultancy | Tailored training |
- Type of course: Manufacturer specific
- Applicable to manufacturer(s): JASCO |
- CPD Approved: Approved
- Scheduled course price: £195.00 + TAX per delegate
- Course discounts: RSC CAMS HEaTED Cambridge Network members receive a discount on this course
Your introduction to advanced liquid chromatography (LC) and liquid chromatography-mass spectrometry (LC-MS) techniques
Module 10 of the Complete LC and LC-MS course and module 2 of The LC and LC-MS Clinic course.
Introduction to advanced LC and LC-MS techniques is approved by the Royal Society of Chemistry (RSC) for the purposes of Continuing Professional Development (CPD). Designed for professionals, students and analytical scientists, this module explores advanced chromatographic and mass spectrometric techniques used to solve challenging analytical problems. As well as covering areas including mass spectral interpretation, multidimensional chromatography and chemometrics to really enhance your knowledge about LC and LC-MS.
This module can be combined with any of the other twelve modules in the Complete LC and LC-MS to tailor your training needs.
Why should I join?
On completion of this module 10 training, you will gain an understanding of advanced analytical strategies used throughout LC and LC-MS, giving you:
- An overview of advanced LC and LC-MS techniques used in modern analytical laboratories
- Understanding of how advanced separation technologies can improve difficult analyses
- Insight into mass spectral interpretation and structural elucidation principles
- Practical guidance on improving chromatographic resolution and sample throughput
- Knowledge of alternative chromatographic techniques beyond conventional reversed-phase LC
- Understanding of specialist detectors and chromatography systems used for complex analytical challenges
What would I learn?
This module focuses on advanced LC and LC-MS methodologies and applications, you will learn:
- The principles of mass spectral interpretation and structural identification using LC-MS data
- How isotopic patterns, fragmentation pathways and accurate mass measurements assist compound identification
- How multidimensional chromatography techniques including heart-cutting LC-LC and comprehensive LC×LC improve resolving power
- The theory and practical applications of hydrophilic interaction chromatography (HILIC)
- How ion-pair chromatography can be used to retain and separate highly polar compounds
- The principles of size exclusion chromatography (SEC/GPC) for molecular weight determinations
- The applications of ion chromatography and ion-exchange chromatography for charged analytes
What knowledge will I gain?
By the end of the module, you will:
- Understand how mass spectral information can be used to identify compounds and interpret molecular structures
- Understand the benefits and limitations of UHPLC and how to successfully transfer existing analytical methods
- Know how to improve chromatographic resolution using advanced separation techniques
- Understand the advantages and limitations of universal and near-universal detector technologies
- Know how advanced detector selection influences sensitivity, selectivity and quantitation
- Understand how advanced column and instrument configurations can significantly improve sample throughput
- Gain insight into modern LC-MS acquisition strategies including data-dependent and data-independent analysis
- Develop an understanding of chemometric techniques including PCA, cluster analysis, SIMCA and PLS modelling
Is this course for me?
This course is designed for anyone who would like to expand their knowledge beyond routine LC and LC-MS analysis.
- Laboratory analysts and technicians seeking advanced LC and LC-MS expertise
- Laboratory managers responsible for analytical strategy and technology selection
- Data analysts interested in advanced interpretation techniques and chemometrics
- Service engineers seeking a deeper understanding of advanced LC and LC-MS applications
- Sales, commercial and marketing teams supporting advanced chromatography and mass spectrometry products and services
If LC and LC-MS touches in your work, this advanced course delivered by experts in the field will equip you with the knowledge required to understand these techniques.
Select the courses you are interested in:
Feedback from previous attendees
“The first part of this module was the most relevant to me, I feel more confident interpreting mass spectra now!” Joe Richardson, Assistant Scientist, Environment Agency (November 2020).
