Practical Essentials of LC introduction mobile phases and pumps and sample introduction
- Duration: 1 day for face-to-face
- Experience level: Intermediate
- Delivery methods: Face-to-face | Offsite training & consultancy | On-Demand | Online | Onsite training & consultancy | Tailored training |
- Instrumentation: Universal
- Manufacturer(s): Agilent | Bruker | CTC | PerkinElmer | Sciex | Scion | Shimadzu | Thermo | Waters |
- CPD Approved: Not yet approved
- Scheduled course price: £385.00 + TAX per delegate
- Course discounts: RSC CAMS Heated members receive a discount on this course
What will you learn on this course?
Day 1 / Modules 1 – 3 of the Complete LC & LC-MS and the Practical Essentials of LC & LC-MS courses.
After an introduction to liquid chromatography, the types of solvents used in mobile phase and their respective properties are examined in detail. This is followed by information on gradient elution (high or low pressure mixing and solvent dwell times); along with problems that are frequently encountered in this area. Strategies for how to optimise your LC system will be discussed, including the necessity to minimise system void volume to increase performance
After lunch, sample introduction techniques are taught in detail, including theory, optimisation and applications. These include 6-port manual injectors; autosamplers; full or partial loop injections; flow through needle injection & column volume compatibility.
Your troubleshooting and maintenance skills will greatly benefit from having a thorough knowledge of inlet systems and how they work.
Combine other days or modules.Learning outcomes
- You will learn the importance of good chromatography.
- You will learn how to select and use the best mobile phase and develop a gradient inlet method.
- You will learn about various autosampler injection techniques and when to use them.
Who is this course for?
If you have limited LC experience, wish to learn about these particular areas or would like to brush-up on your knowledge then this is the course for you.
