Practical Essentials of LC and LC-MS columns, detectors and data analysis
- Duration: 1 day for live courses
- Experience level: Intermediate
- Delivery methods: Face-to-face | Offsite training & consultancy | On-Demand | Online | Onsite training & consultancy | Tailored training |
- Type of course: Universal
- Applicable to manufacturer(s): Agilent | Bruker | CTC analytics | JASCO | PerkinElmer | Sciex | Scion | Shimadzu | Thermo | Waters |
- CPD Approved: Not yet approved
- Scheduled course price: £425.00 + TAX per delegate
- Course discounts: RSC CAMS HEaTED Cambridge Network members receive a discount on this course
Your essential guide to liquid chromatography (LC) and liquid chromatography-mass spectrometry (LC-MS) columns, detectors and data analysis
Day 2 and Modules 4, 5 and 6 of the Complete LC and LC-MS and the Practical Essentials of LC and LC-MS course.
Practical Essentials of LC and LC-MS columns, detectors and data analysis is approved by the Royal Society of Chemistry (RSC) for the purposes of Continuing Professional Development (CPD), and is a classroom-based course that covers analytical columns used in LC and factors that influence chromatographic performance, as well as the various types of detectors used in LC and how they convert analyte separation into analytical data. Then finally, take an in-depth approach to data analysis, covering the principles of data analysis, coupled with qualitative and quantitative approaches used in modern LC and LC-MS laboratories Designed for professionals, students and chemists wanting to take a further step to enhancing their LC and LC-MS knowledge.
This day and modules can be combined with any of the other twelve modules and days in the Complete LC and LC-MS to tailor your training needs.
Why should I join?
On completion of day 2 training, you will gain understanding of analytical column use in LC alongside the various detectors available as well as data processing approaches to LC and LC-MS data, giving you:
- Clear explanations of how LC columns achieve analyte separation
- Understanding of the key factors that influence chromatographic resolution and efficiency
- An overview of the most widely used detectors in LC and LC-MS
- Clear explanations of how different detector types operate
- Understanding of how integration settings influence analytical results
- An overview of calibration methods used for quantitative analysis
- An excellent addition to other modules and days in our Complete LC and LC-MS training
- A great refresher if you have not worked with LC or LC-MS for a while
What would I learn?
This day focuses on columns, detectors and data analysis used in LC and LC-MS, where you will learn:
- How analytes interact with stationary phases to achieve separation
- The key chromatographic parameters that define retention, selectivity, efficiency and resolution
- The principles of chromatographic detection and signal generation
- How detector performance affects sensitivity, selectivity and data quality
- The operation of UV-Vis and photodiode array (PDA) detectors
- How integration algorithms identify and quantify chromatographic peaks
- How to optimise integration parameters for reliable results
- Approaches for analysing co-eluting and overlapping peaks
What knowledge will I gain?
By the end of the day, you will:
- Understand how liquid chromatography systems are classified according to operating pressure
- Know how column chemistry influences analyte retention and selectivity
- Comprehend how particle size affects column efficiency, sensitivity and backpressure
- Understand how LC detectors convert physical and chemical properties into analytical signals
- Know the differences between selective, semi-universal and universal detector technologies
- Know how detector flow cell design can impact chromatographic performance and band broadening
- The fundamentals of chromatographic data acquisition and processing
- How integration algorithms identify and quantify chromatographic peaks
- How to optimise integration parameters for reliable results
Is this course for me?
This day is designed for anyone who would like to learn about columns, detectors and data analysis in LC and LC-MS:
- Laboratory analysts & technicians using LC and LC-MS
- Laboratory or line managers overseeing LC and LC-MS instruments and analysis
- Data analysts and report writers who work with LC and LC-MS data
- Service engineers who want a chemistry focused introduction
- Sales, commercial or marketing teams who work with LC and LC-MS components, instruments, services or personnel in any way and would like more knowledge
If LC and LC-MS touches your work in any way, this well-planned module, delivered by experts in the field, equips you to understand the science behind these analytical chemistry techniques.
