Practical Essentials of LC classification and columns
- Duration: 3.50 hours for on-demand
- Experience level: Intermediate
- Delivery methods: Offsite training & consultancy | On-Demand | Online | Onsite training & consultancy | Tailored training |
- Type of course: Universal
- Applicable to manufacturer(s): Agilent | Bruker | CTC analytics | JASCO | PerkinElmer | Sciex | Scion | Shimadzu | Thermo | Waters |
- CPD Approved: Approved
- Scheduled course price: £227.50 + TAX per delegate
- Course discounts: RSC CAMS HEaTED Cambridge Network members receive a discount on this course
Your essential guide to liquid chromatography (LC) classifications and analytical columns
Module 4 of the Complete LC & LC-MS and the Practical Essentials of LC & LC-MS course.
Practical Essentials of LC classification and columns is approved by the Royal Society of Chemistry (RSC) for the purposes of Continuing Professional Development (CPD). Take an in-depth approach to LC classifications, analytical columns, stationary phase selection and factors that influence chromatographic performance. This LC training is designed for professionals, students and scientists who want to develop a deeper understanding of how columns drive separation performance in LC and LC-MS.
This module can be combined with any of the other twelve modules in the Complete LC & LC-MS to tailor your training needs.
Why should I join?
On completion of this Module 4 training, you will have a solid understanding of LC classification and columns, giving you:
- An overview of the different classifications of LC systems
- Clear explanations of how LC columns achieve analyte separation
- Understanding of the key factors that influence chromatographic resolution and efficiency
- Knowledge of how column dimensions and particle technologies impact performance
- A great addition to the other modules in our Complete LC & LC-MS programme
- A valuable refresher on LC classification an columns and if you have not worked with LC for a while
What would I learn?
This module focuses on explaining the importance of classificaton and columns in LC where you will learn:
- How analytes interact with stationary phases to achieve separation
- The key chromatographic parameters that define retention, selectivity, efficiency and resolution
- How peak shape, band broadening and column performance are related
- The advantages and limitations of different column packing materials
- The differences between normal phase and reversed phase column chemistries
- Best practices for column conditioning, equilibration and care
What knowledge will I gain?
By the end of the module, you will:
- Understand how liquid chromatography systems are classified according to operating pressure
- Know how column chemistry influences analyte retention and selectivity
- Comprehend how particle size affects column efficiency, sensitivity and backpressure
- Understand the role of pore size selection for small molecules, peptides and proteins
- Be familiar with common stationary phases including C18, C8, phenyl, amino, cyano and silica columns
- Understand how column length, internal diameter and flow rate influence chromatographic performance
- Appreciate the importance of guard columns, column heaters and proper column maintenance practices
- Be able to make informed decisions when selecting columns for LC and LC-MS applications
Is this course for me?
This course is designed for anyone who would like to learn about LC column technologies and chromatographic separations.
- Laboratory analysts and technicians using LC and LC-MS systems
- Laboratory or line managers overseeing LC and LC-MS instruments and analytical workflows
- Scientists developing, optimising and validating chromatographic methods
- Researchers seeking a deeper understanding of chromatographic theory and column selection
- Service engineers seeking a chemistry-focused understanding of LC and LC-MS systems
- Sales, commercial or marketing teams who work with LC and LC-MS instruments, columns, consumables or services and would like more in-depth knowledge
If LC or LC-MS touches your work in any way, this well-planned course, delivered by experts in the field, equips you to understand the science behind these techniques.
Select the courses you are interested in:
Feedback from previous attendees
“Good to show generally what different column chemistries are useful for.” Mollie Nero, Project Scientist, Broughton Nicotine Services (February 2021).
“Learning how the choice of column can affect efficiency, resolution and selectivity was a good subject. Also how it is possible to work out how to choose a better column by looking at spectra will potentially help me out in the future.” Joe Richardson, Assistant Scientist, Environment Agency (November 2020).
“[I enjoyed the] level of detail information presented.”
“Useful discussion. Better material and knowledge of the instructor is helpful.”
“Professional, high quality delivery, keep track on time.”
“The instructor is aware of all the LC-MS spectrometry. I found in this course useful materials.”
“[I enjoyed] developing an understanding of the applications and function of the equipment.”
“The HPLC & LC-MS training was good and I enjoyed it because I have learnt a lot.”
