Practical Essentials of LC and LC-MS

- Duration: 3 days for face-to-face 18 hours for Virtual Classroom training
- Experience level: Intermediate
- Delivery methods: Face-to-face | Offsite training & consultancy | On-Demand | Online | Onsite training & consultancy | Tailored training |
- Instrumentation: Universal
- Manufacturer(s): Agilent | Bruker | CTC | PerkinElmer | Sciex | Scion | Shimadzu | Thermo | Waters |
- CPD Approved: Approved
- Scheduled course price: £1,100.00 + TAX per delegate
- Course discounts: RSC CAMS Heated members receive a discount on this course

What will you learn on this course?
This course covers all the practical theory you need to know about liquid chromatography (LC) and its related techniques including liquid chromatography-mass spectrometry (LC-MS); mobile phase selection including gradient elution, pumps and plumbing. Sample introduction using manual injection and autosamplers, sample preparation, calibration standards, sample properties and data analysis.
This course is packed full of examples and practical tips, makes full use of props and includes a Questions and Answers session at the end of each day making it the course to attend for all of your useful LC knowledge!
Learning outcomes
- You will learn the importance of good chromatography.
- You will learn how to select and use the appropriate solvents for your mobile phase.
- You will learn about various autosampler injection methods and when to use each.
- You will learn how to select an appropriate LC column (including stationary phase and column dimensions).
- You will learn the operation and selection of an appropriate LC detector.
- You will learn about the principles and components of the mass spectrometer.
- You will learn about sample preparation techniques and calibration standards.
- You will learn how to undertake data analysis for quantitation and qualitative analysis.
Who is this course for?
This course is for those who have basic knowledge of liquid chromatography and would like to greatly enhance it, learning the practical theory about all available HPLC & LC-MS techniques.
If you would like to improve your knowledge in a particular area and don’t want to attend a 3-day course you can choose to attend individual days from the Practical Essentials of HPLC & LC-MS course.
Course combinations
Attend the LC & LC-MS Clinic course to the end to obtain a full picture and learn Complete LC & LC-MS.
This course includes:
- Practical Essentials of LC introduction mobile phases and pumps and sample introduction
- Practical Essentials introduction to Liquid Chromatography and LC-MS
- Practical Essentials of LC mobile phases and pumps
- Practical Essentials of LC sample introduction
- Practical Essentials of LC and LC-MS columns, detectors and data analysis
- Practical Essentials of LC classification and columns
- Practical Essentials of LC detectors
- Practical Essentials of LC and LC-MS data analysis
- Practical Essentials of LC and LC-MS mass spectrometers and sample preparation
Feedback from previous attendees
“Brilliant, very informative.” Mollie Nero, Project Scientist, Broughton Nicotine Services (February 2021).
“The HPLC & LC-MS training was good and I enjoyed it because I have learnt a lot.” Emmanuella Mambi (November 2019).
“[I enjoyed the] level of detail information presented.” Andrew Bamford, Assistant Scientist, SSCl Environment Agency (May 2019).
“Useful discussion. Better material and knowledge of the instructor is helpful. [Most interesting topic:] How to optimise the method.” Muath Aljeaid, Chemical Engineer, National Company for Mechanical Systems (May 2019).
“The instructor is aware of all the LC-MS spectrometry. I found in this course useful materials.” (May 2019).
“[I enjoyed] developing an understanding of the applications and function of the equipment.” George Bryant, Lab Technician, The Open University (October 2018).