Hands-on HPLC and LC-MS troubleshooting
- Duration: 1 day for live courses
- Experience level: Intermediate
- Delivery methods: Face-to-face | Offsite training & consultancy | Onsite training & consultancy | Tailored training |
- Type of course: Universal
- Applicable to manufacturer(s): Agilent | Bruker | JASCO | PerkinElmer | Sciex | Scion | Shimadzu | Thermo | Waters |
- CPD Approved: Approved
- Scheduled course price: £650.00 + TAX per delegate
- Course discounts: RSC CAMS HEaTED Cambridge Network members receive a discount on this course
Your Hands-on approach to learning high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) troubleshooting
Day 5 of our Hands-on HPLC and LC-MS
Combine the practical theory of HPLC and LC-MS troubleshooting with laboratory-based exercises in this Royal Society of Chemistry (RSC) approved training for the purposes of Continued Professional Development (CPD). This course is designed to bridge the gap between theory and practice, enabling you to confidently diagnose, investigate and resolve problems affecting HPLC and LC-MS instrumentation.
This Hands-on course is ideal for students, analysts and professionals who want to gain practical experience troubleshooting all aspects of HPLC and LC-MS systems including mobile phases, pumps, autosamplers, columns, detectors, vacuum systems, ion sources, mass spectrometers, and data systems. Learn how to apply a logical troubleshooting approach, interpret symptoms, and identify root causes while developing the practical skills needed to restore instrument performance efficiently and effectively.
Why should I join?
On completion, you will have both the theoretical knowledge and practical experience required to confidently troubleshoot HPLC and LC-MS systems in your laboratory.
This Hands-on HPLC & LC-MS troubleshooting course gives you:
- Strong practical skills for diagnosing and resolving HPLC and LC-MS problems
- Practical guidance on identifying issues affecting chromatographic and mass spectrometric performance
- Hands-on experience applying structured troubleshooting methods to HPLC and LC-MS
- An understanding of common faults affecting HPLC and LC-MS instrumentation
- Practical techniques for identifying leaks, contamination, and sensitivity issues
- Experience interpreting chromatograms, mass spectra, and instrument diagnostics
- Confidence investigating the root cause of analytical and instrumental problems
What would I learn?
A user-friendly HPLC and LC-MS troubleshooting course that combines in-depth theory with practical laboratory exercises, you will learn:
- The principles of systematic troubleshooting and fault diagnosis
- How to identify and investigate common chromatographic problems including peak tailing, fronting, splitting, and retention time shifts
- How to diagnose issues associated with mobile phases, solvents, buffers, and sample preparation
- Troubleshooting approaches for pumps, autosamplers, injectors, and fluidic systems
- How to identify column contamination, deterioration, and blockages
- The principles of detector troubleshooting and performance assessment
- How to diagnose vacuum, gas supply, and ion source problems within LC-MS systems
- Approaches to identifying contamination and carryover sources within HPLC and LC-MS instruments
- How to interpret instrument diagnostics, tune reports and performance checks
- Troubleshooting strategies for poor sensitivity, baseline instability, and reproducibility issues
What knowledge will I gain?
- Understand a structured approach to problem solving and root cause analysis in HPLC and LC-MS
- Be confident discussing common instrument faults and their impact on analytical performance
- Understand how contamination, leaks and poor maintenance affect chromatographic and mass spectrometric results
- Explain the symptoms associated with problems in mobile phases, pumps, autosamplers, columns, detectors, vacuum systems, and ion sources
- Recognise the signs of poor chromatographic performance and identify causes
- Understand how contamination can affect sensitivity, baselines, mass spectra, and analytical reliability
- Interpret troubleshooting information from chromatograms, mass spectra, tune reports and instrument diagnostics
- Diagnose common causes of poor sensitivity, poor reproducibility, and instrument instability
- Implement corrective actions and preventative measures to minimise future issues
Is this course for me?
This RSC approved for purposes of CPD training is designed for anyone responsible for operating, maintaining, or supporting HPLC and LC-MS systems, including:
- Analysts & technicians requiring training or a clear structured refresher of HPLC and LC-MS
- Laboratory or line managers overseeing HPLC and LC-MS instrumentation
- Data analysts and report writers who interpret results but do not use the instruments
- Service and consultant engineers who need both a theoretical and hands-on reminder
- Sales, marketing, or commercial teams working with HPLC and LC-MS systems, consumables, data, or laboratory services
If HPLC and LC-MS touches your work in any way, this well-planned course delivered by experts in the field equips you to understand the science behind these techniques.
Course combinations
Can’t attend the full course? No worries, combine days or modules to create training that works best for you. The more days you book the larger the discount.
- For 2 days, pay £1250
- For 3 days, pay £1825
- For 4 days, pay £2350
- For 5 days, pay £2850
Please note, discounts won’t apply when booking but will appear on your invoice.
