Hands-on HPLC and LC-MS
- Duration: 5 days for live courses
- Experience level: Advanced Intermediate
- Delivery methods: Face-to-face | Offsite training & consultancy | Onsite training & consultancy | Tailored training |
- Type of course: Universal
- Applicable to manufacturer(s): Agilent | Bruker | PerkinElmer | Sciex | Scion | Shimadzu | Thermo | Waters |
- CPD Approved: Approved
- Scheduled course price: £2,850.00 + TAX per delegate
- Course discounts: RSC CAMS HEaTED members receive a discount on this course
What will you learn on this course?
This course provides the theory of HPLC and LC-MS essential to any participant, along with the hands-on practical element in the laboratory to practice and re-enforce the theoretical knowledge. These courses are 50% theory and 50% practical in a lab environment.
After an introduction to liquid chromatography, this course addresses solvents & buffers (the mobile phase), pumps, isocratic and gradient elution, sample introduction, analytical columns, HPLC detectors, mass spectrometry including tuning, ionisation, mass analysers, ion detector and data analysis.
The knowledge is then used to create methods, perform injections and change parameters to see the effects. The course discusses the need for maintenance along with practicals to carry out maintenance on both an HPLC and an LC-MS system such as the autosampler, injector, columns, detectors, MS tuning, vacuum system, ion source and the mass analyser. A day is spent on troubleshooting an HPLC and LC-MS instrument and learning what problems can occur, with real problem solving on the instruments and data analysis software.
This course combines the five individual hands-on days covering HPLC Theory and Methods, HPLC Maintenance, LC-MS Theory and Methods, LC-MS Maintenance and HPLC & LC-MS Troubleshooting.
Learning outcomes
- You will learn the importance of good chromatography
- You will learn about the function & operation of the individual HPLC components
- You will become familiar with the HPLC instrumentation
- You will have practical experience of adjusting the parameters for each HPLC component
- You will have practical experience of performing maintenance on each HPLC component
- You will learn the principles of mass spectrometry
- You will learn about the function & operation of the LC-MS individual components
- You will become familiar with the MS instrumentation
- You will have practical experience of adjusting the parameters for each MS component
- You will have practical experience of performing maintenance on each MS component
- You will learn the strategies for HPLC & LC-MS instrument troubleshooting
- You will have practical experience of HPLC & LC-MS instrument troubleshooting
Who is this course for?
This course is suitable for all levels of knowledge; however for complete beginners it is advisable to attend the Absolute Basics of HPLC & LC-MS first to get a good basic overview of liquid chromatography and LC-MS.
Course combinations
Can’t attend the full course? No worries, combine days or modules to create training that works best for you. The more days you book the larger the discount.
- For 2 days, pay £1250
- For 3 days, pay £1825
- For 4 days, pay £2350
- For 5 days, pay £2850
Please note, discounts won’t apply when booking but will appear on your invoice.
This course includes:
Feedback from previous attendees
“The practical skills in LC-MS & the knowledge of MS systems with their functions. It has greatly improved my practical skill and will be very helpful in my work. I would especially recommend the LC-MS instructor, he is super professional. It was a valuable & good experience!” Senior Research Fellow, 2026
“The quality of the information provided. I have worked with HPLC systems for a number of years, but it was embarrassing to realise how much I didn’t know. This course gave me a good understanding of the systems. Furthermore, the amount of info provided on LC-MS was fantastic.” Analytical Chemist, 2026
“Tailored content, with the instructor easily helping problem solve & understand the individual & specialised problems. I’ve only ever trained with Anthias – consistently great knowledge.” QC Shift Analyst II, 2026
