Hands-on HPLC maintenance
- Duration: 1 day for live courses
- Experience level: Intermediate
- Delivery methods: Face-to-face | Offsite training & consultancy | Onsite training & consultancy | Tailored training |
- Type of course: Universal
- Applicable to manufacturer(s): Agilent | JASCO | PerkinElmer | Scion | Shimadzu | Thermo | Waters |
- CPD Approved: Approved
- Scheduled course price: £650.00 + TAX per delegate
- Course discounts: RSC CAMS HEaTED Cambridge Network members receive a discount on this course
Your hands-on approach to learning high-performance liquid chromatography (HPLC) maintenance
Day 2 of our Hands-on HPLC and LC-MS
Combine the practical theory of HPLC maintenance with laboratory-based exercises in this Royal Society of Chemistry (RSC) approved training for the purposes of Continued Professional Development (CPD). This course is designed to bridge the gap between theory and practice, enabling you to confidently maintain HPLC instrumentation within the laboratory.
This Hands-on course is ideal for students, analysts and professionals who want to gain practical experience with all aspects of HPLC maintenance including pumps, autosamplers, injectors, analytical columns, detectors, fittings, tubing, and preventative maintenance procedures. Learn how to identify common performance issues while developing the practical skills needed to maintain reliable, accurate and reproducible HPLC analyses.
Why should I join?
On completion, you will have both the theoretical knowledge and practical experience required to confidently maintain HPLC systems in your laboratory.
This Hands-on HPLC maintenance course gives you:
- Strong practical skills for maintaining HPLC instrumentation
- Practical guidance on maintaining pumps, autosamplers, injectors, columns, and detectors
- Hands-on experience replacing and maintaining common HPLC consumables
- A structured approach to preventative maintenance planning
- Practical techniques for identifying leaks, pressure issues, and performance problems
- Experience maintaining fluidic pathways, fittings, and tubing
- Confidence interpreting maintenance-related performance issues and chromatographic symptoms
What would I learn?
A user-friendly HPLC maintenance course that combines in-depth theory with practical laboratory exercises, you will learn:
- The principles of preventative maintenance for HPLC systems
- How HPLC pumps operate and how to maintain seals, pistons, and check valves
- How to correctly prime pumps, prepare fluidic systems and manage mobile phases
- Routine maintenance procedures for autosamplers, injectors, and sample introduction systems
- How to inspect, replace and maintain needles, rotor seals, sample loops, and associated consumables
- How to minimise system dead volume and maintain chromatographic performance
- Approaches to troubleshooting leaks, blockages, high backpressure, and poor chromatographic performance
What knowledge will I gain?
- Understand the critical role of preventative maintenance in ensuring reliable chromatographic performance
- Be confident discussing HPLC fluidic systems, pump operation, and chromatographic system components
- Understand the impact of leaks, blockages, and dead volume on analytical performance
- Explain the maintenance requirements of pumps, autosamplers, injectors, columns, and detectors
- Understand correct procedures for column storage, conditioning, and regeneration
- Recognise symptoms of column contamination, deterioration, and performance loss before they affect analytical results
- Understand the impact of fittings, tubing, and plumbing configuration on chromatographic efficiency
- Diagnose common causes of pressure instability, poor peak shape, and reduced sensitivity
- Create practical preventative maintenance schedules tailored to laboratory workloads and applications
Is this course for me?
This RSC approved for purposes of CPD training is designed for anyone responsible for maintaining, operating, or supporting HPLC systems, including:
- Analysts & technicians requiring training or a clear structured refresher of HPLC and LC-MS
- Laboratory or line managers overseeing HPLC and LC-MS instrumentation
- Data analysts and report writers who interpret results but do not use the instruments
- Service and consultant engineers who need both a theoretical and hands-on reminder
- Sales, marketing, or commercial teams working with HPLC and LC-MS systems, consumables, data, or laboratory services
If HPLC and LC-MS touches your work in any way, this well-planned course delivered by experts in the field equips you to understand the science behind these techniques.
Course combinations
Can’t attend the full course? No worries, combine days or modules to create training that works best for you. The more days you book the larger the discount.
- For 2 days, pay £1250
- For 3 days, pay £1825
- For 4 days, pay £2350
- For 5 days, pay £2850
Please note, discounts won’t apply when booking but will appear on your invoice.
