Hands-on LC-MS theory and methods
- Duration: 1 day for live courses
- Experience level: Intermediate
- Delivery methods: Face-to-face | Offsite training & consultancy | Onsite training & consultancy | Tailored training |
- Type of course: Universal
- Applicable to manufacturer(s): Agilent | Bruker | JASCO | PerkinElmer | Sciex | Scion | Shimadzu | Thermo | Waters |
- CPD Approved: Approved
- Scheduled course price: £650.00 + TAX per delegate
- Course discounts: RSC CAMS HEaTED Cambridge Network members receive a discount on this course
Your Hands-on approach to learning liquid chromatography-mass spectrometry (LC-MS) theory and methods
Day 3 of our Hands-on HPLC and LC-MS
Combine the practical theory of LC-MS with laboratory-based exercises in this Royal Society of Chemistry (RSC) approved training for the purposes of Continued Professional Development (CPD). This course is designed to bridge the gap between theory and practice, enabling you to confidently use LC-MS instrumentation within the laboratory.
This Hands-on course is ideal for students, analysts and professionals who want to gain practical experience with all aspects of LC-MS including ionisation, mass analysers, tandem mass spectrometry, vacuum systems, data acquisition, qualitative identification, and quantitative analysis. Learn how liquid chromatography and mass spectrometry are combined to provide both chromatographic separation and molecular identification while developing the practical skills needed to create methods, acquire data and interpret results.
Why should I join?
On completion, you will have both the theoretical knowledge and practical experience required to confidently operate LC-MS systems in your laboratory.
This Hands-on LC-MS theory and methods course gives you:
- Strong theoretical and practical skills for using LC-MS instrumentation
- Practical guidance on ionisation techniques, mass analysers, and tandem mass spectrometry
- Hands-on experience creating and optimising LC-MS methods
- An understanding of LC-MS workflows
- Practical experience working with full scan, SIM, and MRM acquisition modes
- Confidence interpreting chromatograms, mass spectra, and fragmentation data
- A structured approach to selecting suitable LC-MS technologies for different applications
What would I learn?
A user-friendly LC-MS course that combines in-depth theory with practical laboratory exercises, you will learn:
- The principles of mass spectrometry and how LC and MS are hyphenated together
- The principles of electrospray ionisation (ESI), atmospheric pressure chemical ionisation (APCI), and atmospheric pressure photoionisation (APPI)
- How ions are generated, transferred, separated, and detected within a mass spectrometer
- The operation and applications of quadrupole, ion trap, time-of-flight (ToF), Orbitrap and tandem quadrupole analysers
- How acquisition parameters influence sensitivity, selectivity, and data quality
- The principles of LC-MS method development and optimisation
- How to perform qualitative identification using accurate mass, isotope patterns, and fragmentation data
- How to perform quantitative analysis using LC-MS data
What knowledge will I gain?
- Understand the complete LC-MS analytical workflow from chromatographic separation through to molecular identification and quantitation
- Be confident discussing LC-MS instrumentation, operating principles, and applications
- Understand how ionisation techniques influence molecular ion formation, adduct formation and fragmentation patterns
- Explain the strengths and limitations of different mass analyser technologies
- Understand the relationship between mass accuracy, mass resolution, sensitivity, and selectivity
- Recognise the importance of vacuum integrity, ion transfer, and detector performance
- Interpret mass spectra, total ion chromatograms, and extracted ion chromatograms
- Understand the principles and limitations of fragmentation-based identification and tandem mass spectrometry
- Select appropriate acquisition modes and analytical strategies for different LC-MS applications
Is this course for me?
This RSC approved for purposes of CPD training is designed for anyone who needs both a theoretical and practical understanding of LC-MS, including:
- Analysts & technicians requiring training or a clear structured refresher of HPLC and LC-MS
- Laboratory or line managers overseeing HPLC and LC-MS instrumentation
- Data analysts and report writers who interpret results but do not use the instruments
- Service and consultant engineers who need both a theoretical and a hands-on reminder
- Sales, marketing, or commercial teams working with HPLC and LC-MS systems, consumables, data, or laboratory services
If HPLC and LC-MS touches your work in any way, this well-planned course delivered by experts in the field equips you to understand the science behind these techniques.
Course combinations
Can’t attend the full course? No worries, combine days or modules to create training that works best for you. The more days you book the larger the discount.
- For 2 days, pay £1250
- For 3 days, pay £1825
- For 4 days, pay £2350
- For 5 days, pay £2850
Please note, discounts won’t apply when booking but will appear on your invoice.
