Practical Essentials of LC and LC-MS sample preparation and sample properties
- Duration: 3.50 hours for on-demand
- Experience level: Intermediate
- Delivery methods: Offsite training & consultancy | On-Demand | Online | Onsite training & consultancy | Tailored training |
- Type of course: Universal
- Applicable to manufacturer(s): Agilent | Bruker | CTC analytics | JASCO | PerkinElmer | Sciex | Scion | Shimadzu | Thermo | Waters |
- CPD Approved: Approved
- Scheduled course price: £227.50 + TAX per delegate
- Course discounts: RSC CAMS HEaTED Cambridge Network members receive a discount on this course
Your essential guide to sample preparation and properties for liquid chromatography (LC) and liquid chromatography-mass spectrometry (LC-MS)
Module 8 of the Complete LC & LC-MS and the Practical Essentials of LC & LC-MS course.
Practical Essentials of LC and LC-MS sample preparation and sample properties is approved by the Royal Society of Chemistry (RSC) for the purposes of Continuing Professional Development (CPD). Take an in-depth approach to sample preparation strategies used in modern LC and LC-MS laboratories, covering sample clean-up, extraction, concentration and optimisation techniques that improve analytical performance and data quality. This training is designed for professionals, students and chemists who want to develop a deeper understanding of how sample preparation and their properties influence analysis.
This module can be combined with any of the other twelve modules in the Complete LC & LC-MS programme to tailor your training needs.
Why should I join?
On completion of this Module 8 training, you will have a solid understanding of sample preparation and properties, giving you:
- An overview of the most widely used sample preparation techniques for LC and LC-MS
- Clear explanations of sample clean-up, extraction and concentration strategies
- Understanding of how sample preparation affects analytical accuracy and precision
- An overview of common sources of analytical error associated with sample handling
- A great addition to the other modules in our Complete LC & LC-MS programme
- A valuable refresher on sample preparation and properties if you have not worked with LC or LC-MS for a while
What would I learn?
This module focuses on explaining the importance of sample preparation and properties in LC and LC-MS, where you will learn:
- Why sample preparation is critical to successful chromatographic analysis
- How filtration, ultrafiltration and desalting techniques protect instruments and improve performance
- How solid phase extraction (SPE) can be used for clean-up, concentration and solvent exchange
- How to optimise SPE methods, including cartridge selection, washing and elution conditions
- The principles of liquid-liquid extraction (LLE) and extraction solvent selection
- Techniques for protein precipitation and biological sample preparation
- How matrix effects influence LC and LC-MS results and strategies to minimise them
What knowledge will I gain?
By the end of the module, you will:
- Understand how sample preparation affects chromatographic performance, detector response and data quality
- Know the benefits and limitations of common sample preparation techniques used in LC and LC-MS
- Be familiar with recovery calculations and the importance of analyte recovery during method validation
- Understand the role of derivatisation in improving analyte detectability, stability and chromatographic behaviour
- Be able to distinguish between reversed phase, normal phase and ion exchange SPE approaches
- Be familiar with matrix matching, internal standards and strategies for reducing matrix effects in LC-MS
- Appreciate the importance of sample solvent selection, injection diluents and mobile phase compatibility
- Understand how sample characteristics such as solubility, stability, polarity and ionisation behaviour influence method development
Is this course for me?
This course is designed for anyone who would like to learn about sample preparation techniques used in LC and LC-MS analysis.
- Laboratory analysts and technicians preparing samples for LC and LC-MS analysis
- Laboratory or line managers overseeing analytical laboratory workflows
- Data analysts and report writers who want to understand the impact of sample preparation on results
- Service engineers seeking a chemistry-focused understanding of sample handling and preparation workflows
- Sales, commercial or marketing teams who work with LC and LC-MS instruments, consumables or services and would like more in-depth knowledge
If LC or LC-MS touches your work in any way, this well-planned course, delivered by experts in the field, equips you to understand the science behind these techniques.
Select the courses you are interested in:
Feedback from previous attendees
“This module was good as it guided us through calibration standards and quality control which gave me confidence that we were doing sufficient quality monitoring already at work.” Joe Richardson, Assistant Scientist, Environment Agency (November 2020).
“[I enjoyed the] level of detail information presented.”
“Useful discussion. Better material and knowledge of the instructor is helpful.”
“Professional, high quality delivery, keep track on time.”
“The instructor is aware of all the LC-MS spectrometry. I found in this course useful materials.”
“[I enjoyed] developing an understanding of the applications and function of the equipment.”
“The HPLC & LC-MS training was good and I enjoyed it because I have learnt a lot.”
