Practical Essentials of LC detectors
- Duration: 1 hour for on-demand
- Experience level: Intermediate
- Delivery methods: Offsite training & consultancy | On-Demand | Online | Onsite training & consultancy | Tailored training |
- Type of course: Universal
- Applicable to manufacturer(s): Agilent | Bruker | CTC analytics | JASCO | PerkinElmer | Sciex | Scion | Shimadzu | Thermo | Waters |
- CPD Approved: Approved
- Scheduled course price: £65.00 + TAX per delegate
- Course discounts: RSC CAMS HEaTED Cambridge Network members receive a discount on this course
Your essential guide to detectors used in liquid chromatography (LC)
Module 5 of the Complete LC & LC-MS and the Practical Essentials of LC & LC-MS course.
Practical Essentials of LC detectors is approved by the Royal Society of Chemistry (RSC) for the purposes of Continuing Professional Development (CPD). Take an in-depth approach to the range of detector technologies used in LC, exploring how detectors convert chromatographic separations into analytical data. This LC training is designed for professionals, students and chemsts who want to enhance their understanding of detector selection, performance and optimisation for LC and LC-MS.
This module can be combined with any of the other twelve modules in the Complete LC & LC-MS programme to tailor your training needs.
Why should I join?
On completion of this Module 5 training, you will have a solid understanding of LC detectors, giving you:
- An overview of the most widely used detectors in LC and LC-MS
- Clear explanations of how different detector types operate
- Understanding of detector selectivity, sensitivity and compatibility considerations
- An overview of detector strengths, limitations and applications
- A great addition to the other modules in our Complete LC & LC-MS programme
- A valuable refresher on LC detector technologies if you have not worked with chromatography for a while
What would I learn?
This module focuses on explaining the importance of detection systems in LC and LC-MS, where you will learn:
- The principles of chromatographic detection and signal generation
- How detector performance affects sensitivity, selectivity and data quality
- The operation of UV-Vis and photodiode array (PDA) detectors
- The fundamentals of fluorescence detection and fluorophore selection
- How refractive index detectors are used for compounds that lack chromophores
- The principles and applications of evaporative light scattering detection (ELSD)
- How charged aerosol detectors (CAD) provide universal detection for non-volatile compounds
- Key considerations when selecting detectors for different analytical applications
What knowledge will I gain?
By the end of the module, you will:
- Understand how LC detectors convert physical and chemical properties into analytical signals
- Know the differences between selective, semi-universal and universal detector technologies
- Know how detector flow cell design can impact chromatographic performance and band broadening
- Understand the advantages and limitations of UV-Vis, PDA, fluorescence, RI, ELSD and CAD detectors
- Be able to assess detector compatibility with analytes, mobile phases and chromatographic methods
- Know the considerations associated with gradient elution and detector performance
- Appreciate when specialised detectors are required for analytes that are not amenable to UV-Vis or mass spectrometric detection
- Be able to make informed detector selection decisions for a wide range of LC and LC-MS applications
Is this course for me?
This course is designed for anyone who would like to learn about detectors used in LC and LC-MS analysis.
- Laboratory analysts and technicians using LC and LC-MS systems
- Laboratory or line managers overseeing LC and LC-MS instruments and analytical workflows
- Service engineers seeking a chemistry-focused understanding of LC detector technologies
- Sales, commercial or marketing teams who work with LC and LC-MS instruments, detectors, consumables or services and would like more in-depth knowledge
If LC or LC-MS touches your work in any way, this well-planned course, delivered by experts in the field, equips you to understand the science behind these techniques.
Select the courses you are interested in:
Feedback from previous attendees
“Good to see different types of detectors and what they are good for.” Mollie Nero, Project Scientist, Broughton Nicotine Services (February 2021).
“This was a short and concise module but gave us lots of useful info about the different types of detectors available and what they are suitable for.” Joe Richardson, Assistant Scientist, Environment Agency (November 2020).
“[I enjoyed the] level of detail information presented.”
“Useful discussion. Better material and knowledge of the instructor is helpful.”
“Professional, high quality delivery, keep track on time.”
“The instructor is aware of all the LC-MS spectrometry. I found in this course useful materials.”
“[I enjoyed] developing an understanding of the applications and function of the equipment.”
“The HPLC & LC-MS training was good and I enjoyed it because I have learnt a lot.”
