Practical Essentials of LC sample introduction
- Duration: 1 hour for on-demand
- Experience level: Intermediate
- Delivery methods: Offsite training & consultancy | On-Demand | Online | Onsite training & consultancy | Tailored training |
- Type of course: Universal
- Applicable to manufacturer(s): Agilent | Bruker | CTC analytics | JASCO | PerkinElmer | Sciex | Scion | Shimadzu | Thermo | Waters |
- CPD Approved: Approved
- Scheduled course price: £65.00 + TAX per delegate
- Course discounts: RSC CAMS HEaTED Cambridge Network members receive a discount on this course
Your essential guide to sample introduction for liquid chromatography (LC)
Module 3 of the Complete LC & LC-MS and the Practical Essentials of LC & LC-MS course.
Practical Essentials of LC sample introduction is approved by the Royal Society of Chemistry (RSC) for the purposes of Continuing Professional Development (CPD). Take an in-depth approach to sample introduction in LC, covering both manual injection techniques and automated sample delivery systems used in modern laboratories. This LC training is designed for professionals, students and chemists who want to enhance their understanding of how samples are introduced into LC systems and how injection performance can impact analytical results.
This module can be combined with any of the other twelve modules in the Complete LC & LC-MS programme to tailor your training needs.
Why should I join?
On completion of this Module 3 training, you will have a solid understanding of sample introduction and autosampler operation in LC and LC-MS, giving you:
- An overview of manual and automated sample introduction techniques
- Clear explanations of injection valves, sample loops and autosampler components
- Understanding of how injection parameters affect analytical performance
- An overview of injection modes and their benefits and limitations
- A great addition to the other modules in our Complete LC & LC-MS programme
- A valuable refresher on sample introduction if you have not worked with LC systems for a while
What would I learn?
This module focuses on explaining the importance of effective sample introduction in LC and LC-MS, where you will learn:
- The key principles of introducing samples into high-pressure LC systems
- How manual injection valves and sample loops operate
- How to select appropriate injection volumes for different applications
- The advantages and limitations of manual injection techniques
- The operation and functionality of modern autosamplers
- The purpose of needle washes, carryover reduction strategies and sample cooling systems
- The different injection modes available in LC autosamplers
What knowledge will I gain?
By the end of the module, you will:
- Understand the challenges associated with introducing samples into high-pressure LC systems
- Know how six-port injection valves operate in both load and inject positions
- Understand the role of sample loops and how loop volume affects chromatographic performance
- Comprehend how injection solvent strength can impact peak shape and separation quality
- Know the benefits of autosamplers for improving reproducibility and sample throughput
- Understand the purpose of weak and strong needle wash solvents in reducing carryover
- Know the differences between full-loop, partial-loop and flow-through needle injection modes
- Know how injection mode selection can affect precision, sample consumption and carryover performance
Is this course for me?
This course is designed for anyone who would like to learn about sample introduction and autosampler technology in LC and LC-MS analysis.
- Laboratory analysts and technicians using LC and LC-MS systems
- Laboratory or line managers overseeing LC and LC-MS instruments and analytical workflows
- Scientists developing and validating chromatographic methods
- Data analysts and report writers working with LC and LC-MS data
- Service engineers seeking a chemistry-focused understanding of sample introduction systems
- Sales, commercial or marketing teams who work with LC and LC-MS instruments, consumables or services and would like more in-depth knowledge
If LC or LC-MS touches your work in any way, this well-planned course, delivered by experts in the field, equips you to understand the science behind these techniques.
Select the courses you are interested in:
Feedback from previous attendees
“[I enjoyed the] level of detail information presented.”
“Useful discussion. Better material and knowledge of the instructor is helpful.”
“Professional, high quality delivery, keep track on time.”
“The instructor is aware of all the LC-MS spectrometry. I found in this course useful materials.”
“[I enjoyed] developing an understanding of the applications and function of the equipment.”
“The HPLC & LC-MS training was good and I enjoyed it because I have learnt a lot.”
