Practical Essentials of mass spectrometers for LC
- Duration: 3.50 hours for on-demand
- Experience level: Intermediate
- Delivery methods: Offsite training & consultancy | On-Demand | Online | Onsite training & consultancy | Tailored training |
- Type of course: Universal
- Applicable to manufacturer(s): Agilent | Bruker | CTC analytics | JASCO | PerkinElmer | Sciex | Scion | Shimadzu | Thermo | Waters |
- CPD Approved: Approved
- Scheduled course price: £227.50 + TAX per delegate
- Course discounts: RSC CAMS HEaTED Cambridge Network members receive a discount on this course
Your essential guide to mass spectrometers for liquid chromatography (LC)
Module 7 of the Complete LC & LC-MS and the Practical Essentials of LC & LC-MS course.
Practical Essentials of mass spectrometers for LC is approved by the Royal Society of Chemistry (RSC) for the purposes of Continuing Professional Development (CPD). Take an in-depth approach to the principles, operation and application of mass spectrometers used in modern LC systems. This training is designed for professionals, students and chemists who want to enhance their understanding of LC instrumentation and mass spectrometric analysis.
This module can be combined with any of the other twelve modules in the Complete LC & LC-MS to tailor your training needs.
Why should I join?
On completion of this Module 7 training, you will have a solid understanding of mass spectrometers used in LC, giving you:
- An overview of the key components of an LC-MS system
- Clear explanations of mass spectrometric principles and terminology
- Understanding of how molecules are ionised, separated and detected
- An overview of the main mass analyser technologies used in LC-MS
- A great addition to the other modules in our Complete LC & LC-MS programme
- A valuable refresher on LC-MS instrumentation if you have not worked with mass spectrometry for a while
What would I learn?
This module focuses on explaining the principles and operation mass spectrometers, where you will learn:
- How liquid chromatography is coupled to mass spectrometry
- The role of vacuum systems in modern mass spectrometers
- The principles of electrospray ionisation (ESI), atmospheric pressure chemical ionisation (APCI) and atmospheric pressure photoionisation (APPI)
- The fundamentals of mass spectra, mass-to-charge ratios (m/z) and isotopic patterns
- The operation of single quadrupole, triple quadrupole, ion trap, Time-of-Flight (ToF) and Orbitrap mass analysers
- The use of SIM, MRM and MS/MS experiments for qualitative and quantitative analysis
- The differences between targeted and non-targeted LC-MS workflows
What knowledge will I gain?
By the end of the module, you will:
- Understand how mass spectrometers generate and interpret mass spectral data
- Know the advantages and limitations of the major ionisation techniques used in LC-MS
- Be familiar with common ion types, adducts and isotope patterns encountered in LC-MS
- Know how vacuum systems and ion optics contribute to instrument performance
- Understand the principles of mass resolution, resolving power and mass accuracy
- Comprehend how tandem mass spectrometry improves sensitivity, selectivity and specificity
- Know how precursor ions and product ions are generated and used in analytical workflows
- Be familiar with the concepts of matrix effects, ion suppression and compensation strategies
Is this course for me?
This course is designed for anyone who would like to learn about mass spectrometry and LC-MS instrumentation.
- Laboratory analysts and technicians using LC-MS systems
- Laboratory or line managers overseeing LC-MS instruments and analytical workflows
- Data analysts and report writers working with LC-MS data
- Service engineers seeking a chemistry-focused understanding of mass spectrometry instrumentation
- Sales, commercial or marketing teams who work with LC-MS instruments, consumables or services and would like more in-depth knowledge
If LC-MS touches your work in any way, this well-planned course, delivered by experts in the field, equips you to understand the science behind these techniques.
Select the courses you are interested in:
Feedback from previous attendees
“[I enjoyed the] level of detail information presented.”
“Useful discussion. Better material and knowledge of the instructor is helpful.”
“Professional, high quality delivery, keep track on time.”
“The instructor is aware of all the LC-MS spectrometry. I found in this course useful materials.”
“[I enjoyed] developing an understanding of the applications and function of the equipment.”
“The HPLC & LC-MS training was good and I enjoyed it because I have learnt a lot.”
