Practical Essentials of LC introduction mobile phases and pumps and sample introduction
- Duration: 1 day for live courses
- Experience level: Intermediate
- Delivery methods: Face-to-face | Offsite training & consultancy | On-Demand | Online | Onsite training & consultancy | Tailored training |
- Type of course: Universal
- Applicable to manufacturer(s): Agilent | Bruker | CTC analytics | JASCO | PerkinElmer | Sciex | Scion | Shimadzu | Thermo | Waters |
- CPD Approved: Not yet approved
- Scheduled course price: £425.00 + TAX per delegate
- Course discounts: RSC CAMS HEaTED Cambridge Network members receive a discount on this course
Your essential introduction to liquid chromatography (LC), mobile phases, LC pumps and sample introduction
Day 1 and Modules 1, 2 and 3 of the Complete LC and LC-MS and the Practical Essentials of LC and LC-MS course.
Practical Essentials of LC introduction, mobile phases, pumps and sample introduction is approved by the Royal Society of Chemistry (RSC) for the purposes of Continuing Professional Development (CPD), and is a classroom-based course that covers the basic definitions of the components involved in LC and LC-MS, the process of separation and the terminology, types of mobile phases and pumps used in LC as well as going into the theory and application of LC sample introduction. Designed for professionals, students and chemists wanting to take the first step to enhancing their LC and LC-MS knowledge.
This day and modules can be combined with any of the other twelve modules and days in the Complete LC and LC-MS to tailor your training needs.
Why should I join?
On completion of day 1 training, you will gain understanding of the fundamentals of LC and LC-MS, as well as the mobile phases, pumps and sample introduction techniques that are applicable to LC, giving you:
- An overview of the LC and LC-MS systems available
- Understanding of how analytes interact with mobile and stationary phases
- Explanations of the properties of the different mobile phases used in LC
- How isocratic and gradient pumping systems influence separation performance
- Understanding of how injection parameters affect analytical performance
- An overview of injection modes and their benefits and limitations
- An excellent start to other modules and days in our Complete LC and LC-MS training
- A great refresher if you have not worked with LC or LC-MS for a while
What would I learn?
This day focuses on introducing LC and LC-MS, mobile phases and pumps as well as sample introduction in LC, where you will learn:
- The principles of liquid chromatography and the stages of chromatographic separation
- How samples, analytes, and matrices influence analytical method development
- How solvent strength influences analyte retention and separation
- How to select appropriate solvents for chromatographic and mass spectrometric applications
- The operation and functionality of modern autosamplers
- The purpose of needle washes, carryover reduction strategies and sample cooling systems
What knowledge will I gain?
By the end of the day, you will:
- Understand the key components that make up an LC and LC-MS system
- Understand how compounds are separated based on their physico-chemical properties
- Know how to prepare, filter and degas mobile phases correctly for reliable analysis
- Be familiar with common LC pump configurations, including reciprocating piston and syringe pump systems
- Know the differences between isocratic and gradient elution methods and when each approach should be used
- Understand the purpose of weak and strong needle wash solvents in reducing carryover
- Know the differences between full-loop, partial-loop and flow-through needle injection modes
- Know how injection mode selection can affect precision, sample consumption and carryover performance
Is this course for me?
This day is designed for anyone who would like an introduction to LC and LC-MS techniques, mobile phases and pumps and LC sample introduction:
- Laboratory analysts & technicians using LC and LC-MS
- Laboratory or line managers overseeing LC and LC-MS instruments and analysis
- Data analysts and report writers who work with LC and LC-MS data
- Service engineers who want a chemistry focused introduction
- Sales, commercial or marketing teams who work with LC and LC-MS components, instruments, services or personnel in any way and would like more knowledge
If LC and LC-MS touches your work in any way, this well-planned module, delivered by experts in the field, equips you to understand the science behind these analytical chemistry techniques.
