Applied UHPLC
- Duration: 1 day for live courses 6.50 hours for on-demand
- Experience level: Intermediate
- Delivery methods: Face-to-face | Offsite training & consultancy | Online | Onsite training & consultancy | Tailored training |
- Type of course: Universal
- Applicable to manufacturer(s): Agilent | JASCO | Thermo | Waters |
- CPD Approved: Approved
- Scheduled course price: £485.00 + TAX per delegate
- Course discounts: RSC CAMS HEaTED Cambridge Network members receive a discount on this course
Your in-depth guide to ultra-high-performance liquid chromatography (UHPLC) with applied training
Go beyond the fundamentals of liquid chromatography with this intermediate 1-day classroom-based UHPLC training for professionals, students and scientists who already have a basic understanding of chromatography and wish to develop a deeper knowledge of UHPLC. Explore the principles, theory, instrumentation, and practical application of UHPLC, including modern UHPLC systems, column technologies, sample introduction, detection, and method optimisation.
This Applied UHPLC training covers the theory and science behind UHPLC alongside the practical considerations required for successful analysis. Discover how UHPLC differs from conventional HPLC, understand the impact of column technology and operating pressure on performance, learn best practices for sample preparation and method development, and gain insight into troubleshooting and maintenance procedures. Reinforce your learning through practical examples and real-world UHPLC applications across a range of industries.
Why should I join?
On completion of this UHPLC training course you will have a more in-depth understanding of UHPLC as a modern analytical separation technique.
This Applied UHPLC course gives you:
- A strong understanding of UHPLC principles and separation mechanisms
- Clear explanations of UHPLC instrumentation and system components
- Understanding of UHPLC column technologies and their influence on performance
- Knowledge of the advantages and limitations of UHPLC compared with HPLC
- A valuable progression from basic chromatography knowledge to UHPLC expertise
- A useful refresher for those returning to UHPLC after a period away from the technique
What would I learn?
This intermediate course focuses on UHPLC theory, instrumentation, method development, column technology, sample preparation, optimisation, maintenance, and troubleshooting. You will learn:
- The principles of liquid chromatography and how UHPLC achieves high-resolution separations
- The differences between low-pressure LC, medium-pressure LC, HPLC and UHPLC systems
- How particle size, column dimensions and operating pressure affect chromatographic performance
- The design and function of key UHPLC components including pumps, autosamplers, columns, detectors, and data systems
- The role of solvent delivery systems and gradient elution in UHPLC separations
- How binary gradient pumps operate and contribute to method performance
- The characteristics and applications of modern UHPLC column technologies
- The importance of sample preparation and suitability for UHPLC analysis
What knowledge will I gain?
By the end of the course, you will:
- Understand the key principles and operational differences between HPLC and UHPLC
- Be able to explain how pressure, flow rate, particle size, and column dimensions influence chromatographic separations
- Have an insight into modern UHPLC instrumentation and be confident discussing the function of each major system component
- Understand the purpose and operation of solvent delivery systems, gradient methods, and sample introduction systems
- Appreciate how column chemistry and technology can be selected to optimise separations
- Understand the relationship between sample composition, matrix effects, and analytical performance
- Be able to understand chromatograms and assess chromatographic quality and performance
- Understand the benefits of low-flow, capillary, and specialty UHPLC columns
- Be familiar with common UHPLC detector technologies and their applications
- Understand routine maintenance requirements and how to prevent common UHPLC problems
Is this course for me?
This UHPLC training is designed for anyone who would like an enhanced understanding of UHPLC
- Laboratory analysts and technicians performing UHPLC or HPLC analyses
- Scientists involved in pharmaceutical, biopharmaceutical, environmental or research testing
- Laboratory managers responsible for chromatography laboratories and analytical workflows
- Data analysts and report writers who work with chromatographic data
- Instrument specialists and service engineers supporting UHPLC systems
- Quality control and quality assurance personnel working with chromatographic methods
- Sales, commercial and marketing professionals involved with chromatography products, instruments, or services
If UHPLC is relevant to your work in any way, this applied course delivered by industry experts will provide you with a deeper understanding of this technique.