Polar stationary phase (silica) & non-polar mobile phase, used for the separation of polar analytes. In normal-phase chromatography, the least polar compounds elute first and the most polar compounds elute last. The mobile phase consists of a non-polar solvent such as hexane or heptane mixed with a slightly more polar solvent such as isopropanol, ethyl acetate or chloroform. Retention increases as the amount of non-polar solvent in the mobile phase increases.
Normal Phase Chromatography Training Courses
Classroom-based training courses:
- 1-day Absolute Basics of LC & LC-MS
- 5-day Complete LC & LC-MS
- 3-day Practical Essentials of LC & LC-MS
- 2-day The LC & LC-MS Clinic
- 1-day Solvents, Pumps, Plumbing and Sample Introduction for LC & LC-MS
- 1-day LC Columns & LC Detectors (including MS)
- 1-day Sample Preparation, Calibration Standards, Sample Properties & Data Analysis
- 1-day LC & LC-MS Method Development, Optimisation & Advanced Techniques
- 1-day LC & LC-MS Troubleshooting and Maintenance