Complete HPLC & LC-MS

Request course outlineBook face-to-face courseBook Virtual Classroom courseArrange course onsiteTailor this courseConsultancy on LC & LC-MSRoyal Society of Chemistry Approved TrainingComplete HPLC & LC-MS - Face-to-Face training | Virtual Classroom training

Course summary

  • Duration: 5 days for face-to-face | Total of 34 hours over 9 days for Virtual Classroom course
  • Experience level: Intermediate
  • Course type: Generic, applicable to all instrumentation
  • Teaching method: Face-to-face OR online via live video, with instrument parts & consumables to see, case studies to solve & chromatograms to troubleshoot
  • RSC CPD approval: Approved for CPD
  • Scheduled course venue: The Open University | Other venues | Virtual Classroom
  • Online/onsite/in-house/offsite availability: Arrange this course just for you
  • Scheduled course price: £1120 + VAT per delegate
  • Course discount: RSC and CAMS members receive a 20% discount on this course in 2020*

Course approval

This course has been approved by the Royal Society of Chemistry for purposes of Continuing Professional Development (CPD).

What will you learn on this course?

The Complete HPLC & LC-MS course is built from the Practical Essentials of HPLC & LC-MS and the HPLC & LC-MS Clinic courses to provide a complete training solution enabling you to understand your Liquid Chromatograph or LC-MS instrument, application method development, troubleshooting & maintenance.

This course covers all the practical theory you need to know about liquid chromatography and its related techniques including liquid chromatography-mass spectrometry.  Mobile phase optimisation including gradient elution, pumps and plumbing, autosampler injection techniques, sample preparation, calibration standards, data analysis for quantitation and qualitative analysis are discussed.

It also looks at how to develop appropriate application specific HPLC & LC-MS methods and the set-up and optimisation of instrumentation.  Finally the steps required to maintain and troubleshoot the system, problem chromatography and mass spectra are covered. Advanced techniques are discussed and their application to difficult samples requiring optimal limits of detection and linear dynamic range.  HPLC to UHPLC method transfer is touched upon, chromatographic deconvolution utilising tandem mass spectrometry, multi-dimensional LC (LCxLC), on-line SPE and chemometrics.

Learning outcomes

  • You will learn the importance of good chromatography.
  • You will learn how to select an appropriate mobile phase and develop gradient elution methods.
  • You will learn about various autosampler injection techniques and when to use them.
  • You will learn how to select an HPLC column (stationary phase and dimensions).
  • You will learn the operation and selection of an appropriate HPLC detector.
  • You will learn about the principles and components of the mass spectrometer.
  • You will learn about sample preparation, calibration standards and sample properties.
  • You will learn how to undertake data analysis for quantitation and qualitatitive analysis.
  • You will learn strategies for HPLC and LC-MS method development.
  • You will learn about system optimisation and configuration for HPLC and LC-MS.
  • You will get an introduction to LC & MS advanced techniques.
  • You will recognise areas requiring maintenance on the instrument.
  • You will learn the logical steps of instrument troubleshooting.

Who is this course for?

The Complete HPLC & LC-MS course is for those who have basic knowledge of liquid chromatography and would like to greatly enhance it, learning the practical theory about all available HPLC & LC-MS techniques.

For complete beginners it is advisable to first attend the 1-day Absolute Basics of HPLC & LC-MS, as you may be overwhelmed by the amount of information on this course.

You can attend the Complete course all at once or, learn about the techniques in the Practical Essentials course, go back to the lab to implement the knowledge and then join us at a later date to attend the more advanced HPLC & LC-MS Clinic course.

If you would like to improve your knowledge in a particular area you can choose to attend individual days and modules from the Complete HPLC & LC-MS course.

Course combinations

If you don't have time to attend the full course all at once, you can learn about the techniques in the Practical Essentials course, go back to the lab to implement the knowledge and then join us at a later date to attend the more advanced HPLC & LC-MS Clinic course.

If you would like to improve your knowledge in a particular area, you can choose to attend individual days. With Virtual Classroom training, you can choose to attend individual modules.