Practical Essentials of HPLC & LC-MS
Course summary
- Duration: 3 days for face-to-face | Total of 18 hours for Virtual Classroom course
- Experience level: Intermediate
- Course type: Universal, applicable to all instrumentation
- Teaching method: Face-to-face OR online via live video, with instrument parts & consumables to see
- RSC CPD approval: Approved for CPD
- Scheduled course venue: The Open University | Other venues | Virtual Classroom
- Online/onsite/in-house/offsite availability: Arrange this course just for you
- Scheduled course price: £1100 + VAT per delegate
- Course discount: RSC and CAMS members receive a discount on this course*
Course approval
This course has been approved by the Royal Society of Chemistry for purposes of Continuing Professional Development (CPD).
What will you learn on this course?
This course covers all the practical theory you need to know about liquid chromatography (LC) and its related techniques including liquid chromatography-mass spectrometry (LC-MS); mobile phase selection including gradient elution, pumps and plumbing. Sample introduction using manual injection and autosamplers, sample preparation, calibration standards, sample properties and data analysis.
This course is packed full of examples and practical tips, makes full use of props and includes a Questions and Answers session at the end of each day making it the course to attend for all of your useful LC knowledge! Add the complementary HPLC & LC-MS Clinic course to the end to obtain a full picture and learn Complete HPLC & LC-MS.
Learning outcomes
- You will learn the importance of good chromatography.
- You will learn how to select and use the appropriate solvents for your mobile phase.
- You will learn about various autosampler injection methods and when to use each.
- You will learn how to select an appropriate LC column (including stationary phase and column dimensions).
- You will learn the operation and selection of an appropriate LC detector.
- You will learn about the principles and components of the mass spectrometer.
- You will learn about sample preparation techniques and calibration standards.
- You will learn how to undertake data analysis for quantitation and qualitative analysis.
Who is this course for?
This course is for those who have basic knowledge of liquid chromatography and would like to greatly enhance it, learning the practical theory about all available HPLC & LC-MS techniques.
If you would like to improve your knowledge in a particular area and don't want to attend a 3-day course you can choose to attend individual days from the Practical Essentials of HPLC & LC-MS course.
Individual Modules - Virtual Classroom training
For Virtual Classroom training, you can choose to attend the individual modules from this course:
- Module 1: Practical Essentials of Liquid Chromatography and LC-MS Introduction
- Module 2: Practical Essentials of Mobile Phases & Pumps in HPLC & LC-MS
- Module 3: Practical Essentials of Sample Introduction in HPLC & LC-MS
- Module 4: Practical Essentials of LC Classification, Columns & Theory
- Module 5: Practical Essentials of LC Detectors
- Module 6: Practical Essentials of Data Analysis for HPLC & LC-MS
- Module 7: Practical Essentials of Mass Spectrometers in LC-MS
- Module 8: Sample Preparation, Calibration Standards and Sample Properties for HPLC & LC-MS
If you have no or very little knowledge of liquid chromatography we recommend that you first attend the 1-day Absolute Basics of HPLC & LC-MS course.
How to book this course
Feedback from previous attendees of Virtual Classroom course
"Brilliant, very informative." Mollie Nero, Project Scientist, Broughton Nicotine Services (February 2021).
Feedback from previous attendees of Face-to-Face course
"The HPLC & LC-MS training was good and I enjoyed it because I have learnt a lot." Emmanuella Mambi (November 2019).
"[I enjoyed the] level of detail information presented." Andrew Bamford, Assistant Scientist, SSCl Environment Agency (May 2019).
"Useful discussion. Better material and knowledge of the instructor is helpful. [Most interesting topic:] How to optimise the method." Muath Aljeaid, Chemical Engineer, National Company for Mechanical Systems (May 2019).
"The instructor is aware of all the LC-MS spectrometry. I found in this course useful materials." (May 2019).
"[I enjoyed] developing an understanding of the applications and function of the equipment." George Bryant, Lab Technician, The Open University (October 2018).